Adamec
Thermocycler and gradient thermocycler
Thermocycler and gradient thermocycler
Place
University: University of South Bohemia in České Budějovice
Faculty: Faculty of Science
Faculty: Department of Parasitology
Street: Branišovská 31
City: České Budějovice
The guarantor
Name and surname: František Adamec, technological scout
Tel.: +420 38777 6262
E-mail:This email address is being protected from spambots. You need JavaScript enabled to view it.
Instrument
Manufacturer: Bio-Rad, Eppendorf, Bioer
Number of pieces: 5 thermocyclers, 1 gradient thermocycler, 2 thermocyclers with interchangeable blocks (gradient and diblocks)
Year of production: 2004-2009
Usage:
Thermocyclers are used in various types of polymerase chain reactions (PCR) for the amplification of specific DNA segments for the purpose of diagnosis, reproduction of DNA for cloning, verifying the presence of the genes / organisms and the like.
Specifications
The laboratory is equipped with a thermocyclers which allow for a wide range of applications. Thanks to gradient blocks and thermocyclers, PCR can be performed in a wide range of temperatures, which allows the optimization of reactions or the performance of different reactions simultaneously.
Manual:
http://www.bioer.com.cn/productcentere/productcentere?id=001002
http://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_10968.pdf
Various
Extern: No
Transfer: No
Terms of lease:
Equipment for 1D and 2D agar and PAGE electrophoresis and electroblotting
Equipment for 1D and 2D agar and PAGE electrophoresis and electroblotting
Place
University: University of South Bohemia in České Budějovice
Faculty: Faculty of Science
Faculty: Department of Parasitology
Street: Branišovská 31
City: České Budějovice
The guarantor
Name and surname: František Adamec, technological scout
Tel.: +420 38777 6262
E-mail:This email address is being protected from spambots. You need JavaScript enabled to view it.
Instrument
Manufacturer: Bio-Rad, Biometra, VWR
Number of pieces:
Year of production: 2005 - 2009
Usage:
Agarose electrophoresis - 1D electrophoresis (RNA, DNA) with the possibility of the analysis of a large number of samples, 2D electrophoresis (using intercalating agents), temperature (TGGE) and denaturing gradient gel electrophoresis (DGGE).
Polyacrylamide electrophoresis - 1D separation of nucleic acids and proteins, the possibility of preparing gradient gels, native electrophoresis, 2D electrophoresis (native / SDS-PAGE, IEF / SDS-PAGE denaturing / SDS-PAGE), isoelectric focusing.
Elektroblotting – tank and semi-dry blotting.
Specifications
The laboratory has a large amount of electrophoretic resources to meet the needs of individual applications. For electrophoretic separation it is possible to use purchased (i.e. Precast) gels and gels prepared in the laboratory including gradient acrylamide gels for SDS-PAGE and native (BN-PAGE). Agarose electrophoresis enables the simultaneous analysis of more than 150 samples (if necessary, one analysis per about half an hour) for the detection of nucleic acids we mainly used SYBR Green (lower toxicological risk), but other detection methods can also be applied. Nucleic acids can be analyzed using denaturing and temperature gradient methods (a more accurate analysis of mutations and supercoiling in circular molecules) and also the RLB method (reverse-line blot). The laboratory is also equipped for the blotting of nucleic acids and their further analysis on membranes. For polyacrylamide electrophoresis (PAGE) single-concentration and gradient gels can be used; we are able to prepare all types of gels thanks to supplementary equipment. Furthermore, isoelectric focusing, denaturing or native electrophoresis in combination with SDS-PAGE as a 2D protein electrophoresis can be used.
Manual:
http://www.bio-rad.com/webroot/web/pdf/lsr/literature/10007296.PDF
http://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_9002.pdf
Various
Extern: No
Transfer: No
Terms of lease:
Liquid chromatography
Liquid chromatography
Place
University: University of South Bohemia in České Budějovice
Faculty: Faculty of Science
Faculty: Department of Parasitology
Street: Branišovská 31
City: České Budějovice
The guarantor
Name and surname: František Adamec, technological scout
Tel.: +420 38777 6262
E-mail:This email address is being protected from spambots. You need JavaScript enabled to view it.
Instrument
Manufacturer: Bio-Rad
Number of pieces: 1
Year of production: 2005
Usage:
The device used to separate the molecules (proteins, nucleic acids, carbohydrates) according to the size or on the basis of affinity using different columns. The column can be used for gel filtration, ion exchange of the column and the affinity column for purification of antibodies, serum proteins and a recombinant protein with a tag (His, STREP). It can be used for analysis and preparation purposes.
Specifications
The possibility of the choice and combination of two different buffers, programmable, isocratic flow and gradient. Fraction collector 10 ml test tube. UV spectrometer and a conductivity meter to monitor the course of chromatography.
Manual:
http://www.bio-rad.com/webroot/web/pdf/WWMSDS/LSGC/USA/USA_USA_7318350.pdf
Various
Extern: No
Transfer: No
Terms of lease:
HPLC - High Performance Liquid Chromatography
HPLC - High Performance Liquid Chromatography
Place
University: University of South Bohemia in České Budějovice
Faculty: Faculty of Science
Faculty: Department of Parasitology
Street: Branišovská 31
City: České Budějovice
The guarantor
Name and surname: František Adamec, technological scout
Tel.: +420 38777 6262
E-mail:This email address is being protected from spambots. You need JavaScript enabled to view it.
Instrument
Manufacturer:
Number of pieces: 1
Year of production: 1995
Usage:
The device used to separate the molecules (proteins, nucleic acids, carbohydrates) according to the size or on the basis of affinity using different columns. The column can be used for gel filtration, ion exchange of the column and the affinity column for purification of antibodies, serum proteins and a recombinant protein with a tag (His, STREP). It can be used for analysis and preparation purposes.
Specifications
The possibility of the choice and combination of three different buffers, programs with an isocratic flow and gradient. Fraction collector (for microtubes and 15-20 ml test tubes). UV spectrometer for monitoring the course during chromatography.
Manual:
Various
Extern: No
Transfer: No
Terms of lease:
Laboratory of Molecular Ecology of Vectors and Pathogens
Laboratory of Molecular Ecology of Vectors and Pathogens
Characteristics
Our laboratory investigates the research of molecular and cellular factors of pathogen transmission by ticks. The research is focused primarily on Lyme disease spirochetes and the tick-borne encephalitis virus. We investigate their interaction with vector tick Ixodes ricinus at the molecular level, protein-carbohydrate interactions and glycemic profiles that affect the transmission of tick-borne pathogens and the immune response of ticks to pathogens. At present, it is also developing research on the molecular ecology and epidemiology of Lyme disease and tick-borne encephalitis using modern methods of diagnosis of these pathogens.
Where to find us?
Contact information
University: University of South Bohemia in České Budějovice
Faculty: Faculty of Science
Faculty: Department of Parasitology
Address:
Faculty of Science,
University of South Bohemia
Branišovská 31
CZ-37005 České Budějovice, CR
Registration No.: 60076658
Tel.: +420605430008
Fax:
E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Web: http://www.prf.jcu.cz/infbiol
Department of Parasitology
Department of Parasitology
Who are we?
The Department of Parasitology ensures the master's and doctoral program of parasitology. The research activity involves structural, ecological, biochemical phylogenetic and molecular aspects of the biology of parasites.
What do we do and for whom?
The department is dedicated to the professional training of students in the field of parasitology. It is targeted into several thematic areas:
Protistan and metazoan parasites in fish
This research has a long tradition associated with fish with several laboratories of the Institute of Parasitology of the ASCR. Most of the topics deal with microsporidia, myxozoa, amphizoic amoebae, tapeworms and parasitic trematodes in fish hosts. The research mainly deals with morphological and ultrastructural characteristics, the description of new species and clarifying their development cycles using techniques of molecular biology.
Medical and Veterinary Parasitology
The research deals with organisms causing so-called opportunistic infections, e.g. cryptosporidia and microsporidia. Using experimental and natural infections, it studies host susceptibility, host specificity, histopathological changes and other important parameters of these diseases.
Molecular biology of carriers
The aim of this theme is to analyze the molecular and cellular factors responsible for the interaction of the carrier with the host. Examples are components of salivary glands of ticks or the molecule α2-macrotubulin as a component of tick immunity. From an ecological and epidemiological point of view the immunological and genetic characterization of different strains of tick-borne encephalitis and Lyme disease agents are especially important.
Molecular phylogenetics and the evolution of parasites
Using molecular information it studies phylogenetic relationships and population structures within parasitic and symbiotic relationships. These analyses serve to clarify various issues of evolutionary parasitology, e.g. the role of host specificity and geographic distribution in the emergence of the parasite population structure or coevolutionary processes between parasites and hosts.
Why do we exist?
The department guarantees the master and doctoral field study program of parasitology. It coordinates the study programs and the qualification thesis of students of bachelor programs focusing on parasitology. It is developing scientific activity in the framework of cooperation between USB FS and the Parasitological Institute BC of the ASCR in the fields of biology and ecology of parasitic protozoa, worms and arthropods and in the fields of biochemistry and molecular biology of parasites.
What are our aims?
The Department of Parasitology will continue the development of existing research activities and will continue to be devoted to training students in the field of parasitology.
Where to find us?
Contact information
Address:
Branišovská 31
České Budějovice
Registration No.: 60076658
Tel.: 387775441
Fax:
E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Web: http://kpa.prf.jcu.cz/
Department of Animal Physiology
Department of Animal Physiology
Who are we?
The Department of Physiology was founded in 2000, originally as part of the USB Faculty of Biology. The main objective of the department is to educate students and their preparation for future practice and coordination of research activities in the field of animal physiology.
What do we do and for whom?
The department offers courses within the specialization of Animal Physiology. The scientific work of the department staff and the students led by them focuses on the study of metabolism and the development of invertebrates and vertebrates and ecological physiology. This study includes research on insect stress hormones and the characterization of their function in triggering anti-stress reactions, physiological research on adaptation to survive low temperatures and the role of circadian oscillators in regulating the daily rhythmicity of behavior and photoperiodism, and metabolism research in aquatic animals.
Why do we exist?
The department coordinates the study programs and dissertations of students of bachelor programs focusing on animal physiology. Furthermore it guarantees master's degree programs in Experimental Biology and the specialization of Animal Physiology and the doctoral field of study Physiology and Developmental Biology of Animals. It is developing its scientific activity in the areas of insect physiology and the fish physiology. It closely cooperates with the Institute of Entomology BC of the ASCR in České Budějovice.
What are our aims?
The aim of the department is to ensure the quality teaching of courses covering important areas of animal physiology, teach students to master the methodological background of the main trends in modern animal physiology and in the research area, to build upon the above mentioned projects. |
Where to find us?
Contact information
Address:
Branišovská 31a,
České Budějovice
Registration No.: 60076658
Tel.:
Fax: +420 385 310 366
E-mail:
Web: http://kfz.prf.jcu.cz/
Processing of maps using GIS tools
Processing of maps using GIS tools
Place
University: University of South Bohemia in České Budějovice
Faculty: Faculty of Science
Department: Department of Ecosystem Biology
Street: Branišovská 31
City: České Budějovice
The guarantor
Name and surname: František Adamec, technological scout
Tel.: +420 38777 6262
E-mail:This email address is being protected from spambots. You need JavaScript enabled to view it.
Specifications
Space relational analysis on units of land use (categories according to specification and data availability)
The selection of interest areas according to the required criteria
On the basis of geographic parameters - slope, exposure, distance from a certain point, place of vision, direction of flow
Based on other parameters – cadastres, settlements
Change of the spatial parameters of the landscape over time (comparison of landscape features based on historical data)
Creation of map outputs from data on the selected territory
Atlas creation
Graphic materials for making land use plans
Creation of master plans, mounts for the selected area
organizing and archiving data, metadata categorization
Sample:
Conditions
We require collateral and the delivery of all supporting materials (maps and data)
Price according to the scope of the request (250 CZK/hour)
Landscape Ecology Laboratory
Landscape Ecology Laboratory
Characteristics
Contact information
University: University of South Bohemia in České Budějovice
Faculty: Faculty of Science
Department: Department of Ecosystem Biology
Address:
Branišovská 31
370 05 České Budějovice
Registration No.: 60076658
Tel.: +420605430008
Fax:
E-mail:This email address is being protected from spambots. You need JavaScript enabled to view it.
Web: http://kbe.prf.jcu.cz/uvod
Department of Ecosystem Biology
Department of Ecosystem Biology
Who are we?
The Department of Ecosystem Biology is a scientific and educational facility that provides instruction in ecological and hydrobiological fields, in which it also carries out research and expert services. Research is carried out in close cooperation with the ASCR institute and is focused on ecosystem processes, with an emphasis on the interaction of plant - soil - water. Graduate theses are drawn up here by students of all levels of study.
What do we do and for whom?
In close cooperation with Šumava NP, we ensure the bachelor’s degree course caring for the environment. Furthermore, together with Biology Centre ASCR, we are developing the field ecosystem biology and we are involved in the postgraduate education of students within the field ecosystem biology and ecosystem hydrobiology.
The main directions of research:
the circulation of substances in the watershed
the function and diversity of microbial communities in water and soil
study of fish communities in reservoirs
the impact of human activities on the ecosystems of wet meadows, peat bogs, mountain spruce forests and water reservoirs
stocks and the bioavailability of organic matter northern tundra soil.
Our research is carried out in the field, but the processing of samples is dependent on the well-equipped laboratories. During the search of causal dependencies, we conduct extensive laboratory experiments. We use traditional methods of ecological research, but also methods of molecular ecology and stable isotopes. Our research, which is conducted in close collaboration with the ASCR Institute and foreign workplaces, is supported by national funding agencies and the European Union.
At the same time we provide expert services in the field of conservation for Šumava National Park and the region of South Bohemia. We also strive to make our results available to the professional and general public.
Why do we exist?
Our main task is to educate in the fields caring for the environment, ecosystem biology and hydrobiology. We place emphasis on understanding the relationships and processes in aquatic and terrestrial ecosystems, and to understand the importance of the role of species diversity. We try to prepare graduates for work in science and research, but also for work in state administration and local governments, in consulting and expertise firms and in the non-profit sector in dealing with environmental issues.
The field caring for the environment is approved as equivalent to the requirements for special professional competence of employees of state and local governments in the field of nature and landscape protection.
The department cooperates with the Institute of Hydrobiology, the Institute of Soil Biology BC ASCR and the Třeboň workplace Institute of Botany in the fields of aquatic ecology and soil organisms, microbial ecology and the study of processes in soil water and wetland ecosystems disturbed by human activities.
What are our aims?
Our goal is to educate students in the field of caring for the environment so that they have a broad knowledge base in biological and environmental subjects and so that they are simultaneously able to understand the practical issues and environmental protection legislation. In all fields, we aim to educate students to be aware of the importance of the interactions in the system plant - soil - water and be able to connect and interpret the findings. We want our students to find jobs at home and abroad. Therefore, our aim is to develop quality research at the national and international level, where students have the opportunity to participate.
Where to find us?
Contact information
Address:
Branišovská 31a,
České Budějovice
Registration No.: 60076658
Tel.: +420 387 772 361
Fax:
E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Web: http://kbe.prf.jcu.cz/uvod