IRMS mass spectrometer
IRMS mass spectrometer
Place
University: University of South Bohemia in České Budějovice
Faculty: Faculty of Science
Faculty: Department of Experimental Plant Biology
Street: Branišovská 31
City: České Budějovice
The guarantor
Name and surname: František Adamec, technological scout
Tel.: +420 38777 6262
E-mail:This email address is being protected from spambots. You need JavaScript enabled to view it.
Instrument
Manufacturer:
Number of pieces: 1
Year of production:
Usage:
Spectrometry is used for determining the isotope ratios of the individual elements in the sample (isotope-ratio mass spectrometry).
Specifications
Here are the prices and the use of the device:
http://kebr.prf.jcu.cz/?act=21
Manual:
Various
Extern: No
Transfer: No
Terms of lease:
HPLC - High Performance Liquid Chromatography
HPLC - High Performance Liquid Chromatography
Place
University: University of South Bohemia in České Budějovice
Faculty: Faculty of Science
Faculty: Department of Parasitology
Street: Branišovská 31
City: České Budějovice
The guarantor
Name and surname: František Adamec, technological scout
Tel.: +420 38777 6262
E-mail:This email address is being protected from spambots. You need JavaScript enabled to view it.
Instrument
Manufacturer:
Number of pieces: 1
Year of production: 1995
Usage:
The device used to separate the molecules (proteins, nucleic acids, carbohydrates) according to the size or on the basis of affinity using different columns. The column can be used for gel filtration, ion exchange of the column and the affinity column for purification of antibodies, serum proteins and a recombinant protein with a tag (His, STREP). It can be used for analysis and preparation purposes.
Specifications
The possibility of the choice and combination of three different buffers, programs with an isocratic flow and gradient. Fraction collector (for microtubes and 15-20 ml test tubes). UV spectrometer for monitoring the course during chromatography.
Manual:
Various
Extern: No
Transfer: No
Terms of lease:
Liquid chromatography
Liquid chromatography
Place
University: University of South Bohemia in České Budějovice
Faculty: Faculty of Science
Faculty: Department of Parasitology
Street: Branišovská 31
City: České Budějovice
The guarantor
Name and surname: František Adamec, technological scout
Tel.: +420 38777 6262
E-mail:This email address is being protected from spambots. You need JavaScript enabled to view it.
Instrument
Manufacturer: Bio-Rad
Number of pieces: 1
Year of production: 2005
Usage:
The device used to separate the molecules (proteins, nucleic acids, carbohydrates) according to the size or on the basis of affinity using different columns. The column can be used for gel filtration, ion exchange of the column and the affinity column for purification of antibodies, serum proteins and a recombinant protein with a tag (His, STREP). It can be used for analysis and preparation purposes.
Specifications
The possibility of the choice and combination of two different buffers, programmable, isocratic flow and gradient. Fraction collector 10 ml test tube. UV spectrometer and a conductivity meter to monitor the course of chromatography.
Manual:
http://www.bio-rad.com/webroot/web/pdf/WWMSDS/LSGC/USA/USA_USA_7318350.pdf
Various
Extern: No
Transfer: No
Terms of lease:
Equipment for 1D and 2D agar and PAGE electrophoresis and electroblotting
Equipment for 1D and 2D agar and PAGE electrophoresis and electroblotting
Place
University: University of South Bohemia in České Budějovice
Faculty: Faculty of Science
Faculty: Department of Parasitology
Street: Branišovská 31
City: České Budějovice
The guarantor
Name and surname: František Adamec, technological scout
Tel.: +420 38777 6262
E-mail:This email address is being protected from spambots. You need JavaScript enabled to view it.
Instrument
Manufacturer: Bio-Rad, Biometra, VWR
Number of pieces:
Year of production: 2005 - 2009
Usage:
Agarose electrophoresis - 1D electrophoresis (RNA, DNA) with the possibility of the analysis of a large number of samples, 2D electrophoresis (using intercalating agents), temperature (TGGE) and denaturing gradient gel electrophoresis (DGGE).
Polyacrylamide electrophoresis - 1D separation of nucleic acids and proteins, the possibility of preparing gradient gels, native electrophoresis, 2D electrophoresis (native / SDS-PAGE, IEF / SDS-PAGE denaturing / SDS-PAGE), isoelectric focusing.
Elektroblotting – tank and semi-dry blotting.
Specifications
The laboratory has a large amount of electrophoretic resources to meet the needs of individual applications. For electrophoretic separation it is possible to use purchased (i.e. Precast) gels and gels prepared in the laboratory including gradient acrylamide gels for SDS-PAGE and native (BN-PAGE). Agarose electrophoresis enables the simultaneous analysis of more than 150 samples (if necessary, one analysis per about half an hour) for the detection of nucleic acids we mainly used SYBR Green (lower toxicological risk), but other detection methods can also be applied. Nucleic acids can be analyzed using denaturing and temperature gradient methods (a more accurate analysis of mutations and supercoiling in circular molecules) and also the RLB method (reverse-line blot). The laboratory is also equipped for the blotting of nucleic acids and their further analysis on membranes. For polyacrylamide electrophoresis (PAGE) single-concentration and gradient gels can be used; we are able to prepare all types of gels thanks to supplementary equipment. Furthermore, isoelectric focusing, denaturing or native electrophoresis in combination with SDS-PAGE as a 2D protein electrophoresis can be used.
Manual:
http://www.bio-rad.com/webroot/web/pdf/lsr/literature/10007296.PDF
http://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_9002.pdf
Various
Extern: No
Transfer: No
Terms of lease:
Thermocycler and gradient thermocycler
Thermocycler and gradient thermocycler
Place
University: University of South Bohemia in České Budějovice
Faculty: Faculty of Science
Faculty: Department of Parasitology
Street: Branišovská 31
City: České Budějovice
The guarantor
Name and surname: František Adamec, technological scout
Tel.: +420 38777 6262
E-mail:This email address is being protected from spambots. You need JavaScript enabled to view it.
Instrument
Manufacturer: Bio-Rad, Eppendorf, Bioer
Number of pieces: 5 thermocyclers, 1 gradient thermocycler, 2 thermocyclers with interchangeable blocks (gradient and diblocks)
Year of production: 2004-2009
Usage:
Thermocyclers are used in various types of polymerase chain reactions (PCR) for the amplification of specific DNA segments for the purpose of diagnosis, reproduction of DNA for cloning, verifying the presence of the genes / organisms and the like.
Specifications
The laboratory is equipped with a thermocyclers which allow for a wide range of applications. Thanks to gradient blocks and thermocyclers, PCR can be performed in a wide range of temperatures, which allows the optimization of reactions or the performance of different reactions simultaneously.
Manual:
http://www.bioer.com.cn/productcentere/productcentere?id=001002
http://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_10968.pdf
Various
Extern: No
Transfer: No
Terms of lease:
Biohazard flow-box
Biohazard flow-box
Place
University: University of South Bohemia in České Budějovice
Faculty: Faculty of Science
Faculty: Department of Parasitology
Street: Branišovská 31
City: České Budějovice
The guarantor
Name and surname: František Adamec, technological scout
Tel.: +420 38777 6262
E-mail:This email address is being protected from spambots. You need JavaScript enabled to view it.
Instrument
Manufacturer: Telstar
Number of pieces: 2
Year of production: 2007, 2010
Usage:
Allows you to work in a clean environment, prevents the contamination of samples and also protects workers. Suitable for working with infectious and highly infectious material, also for the processing of any contamination-sensitive samples.
Specifications
Flow-box is equipped with HEPA filtration intake and exhaust air (HEPA 99.999% efficiency), a motorized window with a seal, UV sterilization, a gas burner, a backup battery for closing the window in case of a power failure, electronic control and online monitoring of the functionality of the HEPA filters and the other parts of the box.
Manual:
Various
Extern: No
Transfer: No
Terms of lease:
Homogeniser
Homogeniser
Place
University: University of South Bohemia in České Budějovice
Faculty: Faculty of Science
Faculty: Department of Parasitology
Street: Branišovská 31
City: České Budějovice
The guarantor
Name and surname: František Adamec, technological scout
Tel.: +420 38777 6262
E-mail:This email address is being protected from spambots. You need JavaScript enabled to view it.
Instrument
Manufacturer: Qiagen
Number of pieces: 1
Year of production: 2009
Usage:
Enables the standardised homogenisation of biological samples.
Specifications
The homogenizer operates with a frequency of 30 shakes per second, homogenization takes place using autoclavable metal balls, with the possibility of the simultaneous homogenisation of 48 samples. In case of need it is also possible to homogenize under reduced temperatures. Time of homogenization is up to 5 minutes.
Manual:
http://www.qiagen.com/literature/render.aspx?id=200069
Various
Extern: No
Transfer: No
Terms of lease:
Incubators
Incubators
Place
University: University of South Bohemia in České Budějovice
Faculty: Faculty of Science
Faculty: Department of Parasitology
Street: Branišovská 31
City: České Budějovice
The guarantor
Name and surname: František Adamec, technological scout
Tel.: +420 38777 6262
E-mail:This email address is being protected from spambots. You need JavaScript enabled to view it.
Instrument
Manufacturer: Nüve
Number of pieces: 4
Year of production: 1980-2010
Usage:
Cultivation of cell cultures / tissues and carrying out the reactions under stable temperature conditions. The possibility of using CO2 sealed plastic boxes for cultivating cell cultures requiring more demanding conditions.
Specifications
The laboratory has 3 incubators without refrigeration options (manual and electronic control) and 1-cooled incubator (electronic control units).
Manual:
http://www.nuve.com.tr/images/urun/pdf/es_120_-_new.pdf
Various
Extern: No
Transfer: No
Terms of lease:
PCR boxes
PCR boxes
Place
University: University of South Bohemia in České Budějovice
Faculty: Faculty of Science
Faculty: Department of Parasitology
Street: Branišovská 31
City: České Budějovice
The guarantor
Name and surname: František Adamec, technological scout
Tel.: +420 38777 6262
E-mail:This email address is being protected from spambots. You need JavaScript enabled to view it.
Instrument
Manufacturer:
Number of pieces: 2
Year of production: 2008, 2009
Usage:
Preparation of PCR reactions in clean conditions, thereby preventing contamination and false-positive results.
Specifications
Equipped with UV sterilization, HEPA filtration of intake air.
Manual:
Various
Extern: No
Transfer: No
Terms of lease:
Basic laboratory equipment
Basic laboratory equipment
Place
University: University of South Bohemia in České Budějovice
Faculty: Faculty of Science
Faculty: Department of Parasitology
Street: Branišovská 31
City: České Budějovice
The guarantor
Name and surname: František Adamec, technological scout
Tel.: +420 38777 6262
E-mail:This email address is being protected from spambots. You need JavaScript enabled to view it.
Instrument
Manufacturer: Labnet, Bioer, Eppendorf, Gilson, Orange Scientific, Hettich, VWR
Number of pieces:
Year of production: 2000-2010
Usage:
Basic equipment for the preparation and processing of samples.
Specifications
The basic equipment consists of small appliances and other equipment necessary for work in the laboratory such as automatic pipettes, pipettors, centrifuges, vortexes (including attachments), shakers, pH meters, scales and analytical balances, sonicators, ultrasonic baths, dry blocks, shaking dry blocks, hybridization ovens and others.
Manual:
Various
Extern: No
Transfer: No